The present study qantitates the in vitro cytotoxicity of a variety of denture base acrylic resins using cell culture techniques combined with image analysis to measure nuclear area and DNA contents. In this study, a comparison was made among direct curing, heat curing and microwave curing resins.
The results obtained from this study were as follows :
1. Morphologically, cell process and nucleus became prominent but macroscopic difference according to the resins were not observed In addition, increased cellular density around the specimen were observed.
2. In DNA contents measurements, S -- G2M phase cell was 15A7%, 14.58% in control and heat curing resin on 1st day and the others group 21.39-3336% were measured.
3. Nuclear area and DNA contents were increased on 3rd day except DNA content of the microwave curing resin group.
These results suggest that denture base acrylic resins stimulate gingival fibroblasts in vitro, especially stimulation of direct curing resin is larger and longer than the others.
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